JOURNAL ARTICLE

Crystallization of Pleurotus ostreatus (oyster mushroom) lectin

T. ChattopadhyayJ. N. LisgartenRolf BrechtelHarold RüdigerRex A. Palmer

Year: 1999 Journal:   Acta Crystallographica Section D Biological Crystallography Vol: 55 (9)Pages: 1589-1590   Publisher: Wiley

Abstract

Crystals of Pleurotus ostreatus (oyster mushroom) lectin have been grown by the hanging-drop technique using ammonium sulfate as the precipitant at 293 K. Over a period of between two and three weeks, crystals of hexagonal bipyramidal morphology grew to maximum dimensions of 0.2 × 0.2 × 0.5 mm. The crystals belong to space group P 6 1 22 or P 6 5 22, with unit-cell parameters a = b = 155.9, c = 149.8 Å, V = 3153078 Å 3 , Z = 12 (assuming 50% solvent), and diffract to 4.1 Å at 293 K.

Keywords:
Mushroom Pleurotus ostreatus Crystallization Oyster Ammonium sulfate Lectin Bipyramid Chemistry Solvent Crystallography Biology Food science Crystal structure Chromatography Biochemistry Fishery Organic chemistry

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0.39
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Citation History

Topics

Glycosylation and Glycoproteins Research
Life Sciences →  Biochemistry, Genetics and Molecular Biology →  Molecular Biology
Carbohydrate Chemistry and Synthesis
Physical Sciences →  Chemistry →  Organic Chemistry
Polysaccharides and Plant Cell Walls
Life Sciences →  Agricultural and Biological Sciences →  Plant Science

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