Amperometric Glucose Biosensor Based on Pt‐Pd Nanoparticles Supported by Reduced Graphene Oxide and Integrated with Glucose Oxidase

Abstract

Abstract A novel glucose biosensor was developed based on the immobilization of glucose oxidase (GOx) on reduced graphene oxide incorporated with electrochemically deposited platinum and palladium nanoparticles (PtPdNPs). Reduced graphene oxide (RGO) was more hybridized by chemical and heat treatment. Bimetallic nanoparticles were deposited electrochemically on the RGO surface for potential application of the PdPt alloy in biosensor preparation. The as‐prepared hybrid electrode exhibited high electrocatalytic activities toward H 2 O 2 , with a wide linear response range from 0.5 to 8 mM ( R 2 =0.997) and high sensitivity of 814×10 −6 A/mMcm 2 . Furthermore, glucose oxidase with active material was integrated by a simple casting method on the RGO/PdPtNPs surface. The as‐prepared biosensor showed good amperometric response to glucose in the linear range from 2 mM to 12 mM, with a sensitivity of 24×10 −6 A/mMcm 2 , a low detection limit of 0.001 mM, and a short response time (5 s). Moreover, the effect of interference materials, reproducibility and the stability of the sensor were also investigated.