Fabrication of Microfluidic Devices for Forensic Molecular Diagnostics

Abstract

There have been considerable advances in the development of microfluidic devices that can carry out the automated and sophisticated processing necessary for DNA analysis and forensic biology. Polymer substrates with high replication techniques are often used in the manufacture of microfluidic devices that are single use, low cost and disposable. High replication techniques require the creation of a master mould from which the devices are subsequently fabricated. This review will describe the use of high replication techniques for device fabrication, with a focus on creation of the master mould manufacture and application of these devices in molecular diagnostics for forensic applications.