Immobilization of Photosynthetic Reaction Center Complexes onto a Hydroquinonethiol-Modified Gold Electrode

Abstract

Abstract The immobilization of reaction center complexes onto a gold electrode was attempted through the binding affinity between reaction centers and hydroquinone-2-thiol adsorbed on an electrode surface. The largest anodic photoresponse by immobilized reaction centers was observed using ubiquinone B-depleted reaction centers and with the pre-oxidation of adsorbed hydroquinone-2-thiol, indicating that the degree of the orientation of reaction center particles increases through the binding affinity between vacant QB-sites in the particles and p-benzoquinonethiol. This anodic photoresponse could be elongated over a period of 5 h (2500 cycles of turnover number) by adding cytochrome c2 into the electrolytic solution.