Flow injection chemiluminescence determination of naphazoline hydrochloride in pharmaceuticals

Abstract

ABSTRACT A simple and sensitive flow injection chemiluminescence (FI‐CL) method was developed for the determination of naphazoline hydrochloride (NPZ). The method is based on the enhancing effect of NPZ on the weak CL signal from the reaction of KIO 4 with H 2 O 2 . Experimental parameters that affected the CL signal, including the pH of the KIO 4 solution, concentrations of KIO 4 , H 2 O 2 and disodium‐EDTA and flow rate were optimized. Under the optimum conditions, the increment of CL intensity was linearly proportional to the concentration of NPZ in the range 5.0 × 10 −6 to 70 × 10 −6 mol/L. The detection limit was 1.0 × 10 −6 mol/L and the relative standard deviation for 50 × 10 −6 mol/L NPZ solution was 2.8% ( n = 11). In addition, a high throughput of 120 samples/h was achieved. The utility of this method was demonstrated by determining NPZ in pharmaceuticals. Copyright © 2013 John Wiley & Sons, Ltd.