JOURNAL ARTICLE

Phosphorylation-regulated crosslinking of gold nanoparticles: a new strategy for colorimetric detection of protein kinase activity

Sujuan SunHaixia ShenChenghui LiuZhengping Li

Year: 2015 Journal:   The Analyst Vol: 140 (16)Pages: 5685-5691   Publisher: Royal Society of Chemistry

Abstract

A facile colorimetric protein kinase assay has been developed based on the peptide phosphorylation-tuned crosslinking and aggregation of gold nanoparticles.

Keywords:
Biotinylation Streptavidin Colloidal gold Chemistry Protein kinase A Peptide Phosphorylation Biotin Kinase Biochemistry Serine Nanoparticle Biophysics Nanotechnology Biology Materials science

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23
Cited By
1.59
FWCI (Field Weighted Citation Impact)
37
Refs
0.84
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Is in top 1%
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Citation History

Topics

Advanced biosensing and bioanalysis techniques
Life Sciences →  Biochemistry, Genetics and Molecular Biology →  Molecular Biology
Advanced Biosensing Techniques and Applications
Life Sciences →  Biochemistry, Genetics and Molecular Biology →  Molecular Biology
Advanced Proteomics Techniques and Applications
Physical Sciences →  Chemistry →  Spectroscopy
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