免疫比濁法による IgA 及び IgM の測定

Abstract

By using a Monarch centrifugal analyser, we evaluated a turbidimetric assay for IgA and IgM concentrations in serum. Studies of within-run precision using serum gave CV's of 3.9% at 71.1mg/d1, 1.7% at 240.0mg/d1 and 1.8% at 440.2mg/dl for IgA and 2.3% at 47.4 mg/dl, 5.5% at 116.2mg/d1 and 3.6% at 312.2mg/d1 for IgM (n =20). Interferences due to hemolysis, icterum and lipemia were studied using 0 —1000mg/d1 hemoglobin, 0 —100mg/d1 bilirubin and 0 — 5 % intralipid. At these levels, these tests showed almost no effects. The tur¬bidimetric immunoassay was compared with a laser nephelometric assay (Behring Nephelo¬meter) by analysis of sera obtained from in-patients. Regression analysis showed good correla¬tion between these assays (IgA: n=97, r =0.965, y =0.83 +0.60, IgM: n =93, r =0.950, y =1.06 +11.9). We conclude that the turbidimetric immunoassay using Monarch centrifugal analyser produces precise results and is convenient for the measurements of immunoglobulins.