Hybridization and Detection of Fluorescein-Labeled DNA Probes Using Enhanced Chemiluminescence

Abstract

DNA probes can be labeled using the modified nucleotide, fluo-rescein-11-dUTP, and subsequently detected with enhanced chemiluminescence (1) using an antifluorescein antibody conjugated to horseradish peroxidase (2). The use of enhanced chemiluminescence avoids the fading of results associated with some colormetric detection procedures, used with nonradioactive labeling and detection systems. Hard copy results showing excellent resolution are produced.